Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
1.
Biomater Sci ; 12(7): 1847-1863, 2024 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-38411258

RESUMO

Axon regeneration and Schwann cell proliferation are critical processes in the repair and functional recovery of damaged neural tissues. Biomaterials can play a crucial role in facilitating cell proliferative processes that can significantly impact the target tissue repair. Chemical decellularization and supercritical fluid-based decellularization methods are similar approaches that eliminate DNA from native tissues for tissue-mimetic biomaterial production by using different solvents and procedures to achieve the final products. In this study, we conducted a comparative analysis of these two methods in the context of nerve regeneration and neuron cell differentiation efficiency. We evaluated the efficacy of each method in terms of biomaterial quality, preservation of extracellular matrix components, promotion of neuronal cell differentiation and nerve tissue repair ability in vivo. Our results indicate that while both methods produce high-quality biomaterials, supercritical fluid-based methods have several advantages over conventional chemical decellularization, including better preservation of extracellular matrix components and mechanical properties and superior promotion of cellular responses. We conclude that supercritical fluid-based methods show great promise for biomaterial production for nerve regeneration and neuron cell differentiation applications.


Assuntos
Regeneração Nervosa , Tecido Nervoso , Matriz Extracelular/química , Axônios , Materiais Biocompatíveis/química , Engenharia Tecidual/métodos , Tecidos Suporte/química
2.
J Control Release ; 368: 140-156, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38373473

RESUMO

Traumatic brain injuries(TBI) pose significant challenges to human health, specifically neurological disorders and related motor activities. After TBI, the injured neuronal tissue is known for hardly regenerated and recovered to their normal neuron physiology and tissue compositions. For this reason, tissue engineering strategies that promote neuronal regeneration have gained increasing attention. This study explored the development of a novel neural tissue regeneration cryogel by combining brain-derived decellularized extracellular matrix (ECM) with heparin sulfate crosslinking that can perform nerve growth factor (NGF) release ability. Morphological and mechanical characterizations of the cryogels were performed to assess their suitability as a neural regeneration platform. After that, the heparin concnentration dependent effects of varying NGF concentrations on cryogel were investigated for their controlled release and impact on neuronal cell differentiation. The results revealed a direct correlation between the concentration of released NGF and the heparin sulfate ratio in cryogel, indicating that the cryogel can be tailored to carry higher loads of NGF with heparin concentration in cryogel that induced higher neuronal cell differentiation ratio. Furthermore, the study evaluated the NGF loaded cryogels on neuronal cell proliferation and brain tissue regeneration in vivo. The in vivo results suggested that the NGF loaded brain ECM derived cryogel significantly affects the regeneration of brain tissue. Overall, this research contributes to the development of advanced neural tissue engineering strategies and provides valuable insights into the design of regenerative cryogels that can be customized for specific therapeutic applications.


Assuntos
Lesões Encefálicas Traumáticas , Engenharia Tecidual , Humanos , Encéfalo , Lesões Encefálicas Traumáticas/terapia , Criogéis , Matriz Extracelular , Heparina , Fator de Crescimento Neural/farmacologia , Regeneração Nervosa , Sulfatos , Engenharia Tecidual/métodos
3.
Mater Today Bio ; 23: 100881, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38161511

RESUMO

Wound healing is a critical process that facilitates the body's recovery from injuries and helps prevent infections, thereby maintaining overall tissue and organ functionality. However, delayed wound healing owing to various factors can lead to bacterial infections and secondary complications. In this study, a ciprofloxacin (CIP)-loaded MXene/sodium alginate (SA) hydrogel was fabricated to inhibit bacterial infections and enhance wound healing. The hydrogel was formulated in a sprayable state by blending CIP-loaded MXene (CIP-MX) with SA. This hydrogel was found to exhibit excellent photothermal conversion capability and biocompatibility under near-infrared (NIR) irradiation. In addition, the hydrogel enabled controlled drug release based on NIR irradiation, ultimately enabling improved antibacterial activity. Based on the in vitro and in vivo experiments, the CIP-loaded MXene/SA hydrogel (CIP-MX@Gel) accelerated wound healing. Overall, the CIP-MX@Gel has excellent potential as an effective wound healing material.

4.
Macromol Biosci ; 21(8): e2100160, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34121330

RESUMO

Supercritical fluid-based extraction technologies are currently being increasingly utilized in high purity extract products for food industries. In recent years, supercritical fluid-based extraction technology is transformed in biomaterials process fields to be further utilized for tissue engineering and other biomedical applications. In particular, supercritical fluid-based decellularization protocols have great advantage over the conventional decellularization as it may allow preservation of extracellular matrix components and structures. In this review, the latest technological development utilizing the supercritical fluid-based decellularization for regenerative medicine is introduced.


Assuntos
Matriz Extracelular , Medicina Regenerativa , Materiais Biocompatíveis , Matriz Extracelular/química , Medicina Regenerativa/métodos , Tecnologia , Engenharia Tecidual/métodos , Tecidos Suporte/química
5.
J Clin Pathol ; 71(9): 774-780, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29559518

RESUMO

AIMS: Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) are clinically different, and the rapid detection and differentiation of M. tuberculosis complex (MTBC) and NTM is crucial for patient management and infection control. Given the slow growth of most pathogenic mycobacteria, nucleic acid amplification assays are excellent tools for direct identification of mycobacteria in clinical specimens. Recently, a multiplex real-time PCR assay was developed that can directly detect 20 mycobacterial species in clinical specimens. Here, we evaluated the diagnostic performance of the assay for diagnosing mycobacterial disease under routine laboratory conditions. METHODS: A total of 3334 specimens collected from 1437 patients suspected of tuberculosis infection were subjected to acid-fast bacilli staining, conventional culture and the multiplex real-time PCR assay. To evaluate the sensitivity and specificity of the assay, the overall diagnosis of tuberculosis was defined by positive culture plus medical history, and the 2007 American Thoracic Society and Infectious Disease Society of America diagnostic criteria for NTM disease were applied. RESULTS: The sensitivity, specificity, positive predictive value and negative predictive value were 87.5%, 99.6%, 96.1% and 98.5%, respectively, for the detection of MTBC isolates and 53.3%, 99.9%, 95.2%, and 98.9%, respectively, for detecting NTM isolates. CONCLUSIONS: Thus, the assay can correctly differentiate between MTBC and NTM isolates in clinical specimens and would be a useful tool for the rapid differentiation of tuberculosis and NTM disease, despite its limited sensitivity for the diagnosis of NTM disease.


Assuntos
Técnicas Bacteriológicas , DNA Bacteriano/genética , Reação em Cadeia da Polimerase Multiplex , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Mycobacterium tuberculosis/genética , Micobactérias não Tuberculosas/genética , Tuberculose/diagnóstico , DNA Bacteriano/isolamento & purificação , Diagnóstico Diferencial , Humanos , Infecções por Mycobacterium não Tuberculosas/microbiologia , Mycobacterium tuberculosis/isolamento & purificação , Micobactérias não Tuberculosas/isolamento & purificação , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Tuberculose/microbiologia , Fluxo de Trabalho
6.
Neurosci Lett ; 628: 230-5, 2016 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-27345382

RESUMO

Recently, the effect of genetic variants in the Vitamin D receptor (VDR) gene on Parkinson's disease (PD) has gained interest. However, the precise relationship between VDR polymorphisms and PD remains unclear. In Korea, one study reported an association between VDR gene polymorphisms and PD. However, this study was conducted with a small sample size, and only the Bsml locus was evaluated. Therefore, further investigations about the relationship between VDR polymorphisms and PD are necessary in a Korean population. A total of 300 subjects were included in this study. One hundred and forty-six PD patients were diagnosed according to the United Kingdom Parkinson's Disease Society Brain Bank (UKPDBB) criteria with abnormal dopamine transporter imaging, and 154 healthy control subjects were also enrolled. We used a TaqMan genotyping assay to identify four SNPs of the VDR gene, including BsmI, FokI, ApaI, and TaqI (rs731236, rs2228570, rs7976091, and rs731236). A significant association was not noted between the risk of PD and genetic polymorphisms in the four loci in a Korean population. However, when the genetic variants of the VDR gene were analyzed after adjusting for the serum 25-OH vitamin D3 level, the TaqI and BsmI minor allele increased the risk of PD. Our data suggest no correlation between PD and the VDR polymorphisms, including BsmI, FokI, ApaI, and TaqI, in a Korean population; however, the results should be interpreted carefully because gene-environment interactions may exist. Further investigations of the VDR and its relationship with PD are required to identify the role of vitamin D in the pathogenesis of PD.


Assuntos
Doença de Parkinson/genética , Polimorfismo de Nucleotídeo Único , Receptores de Calcitriol/genética , Idoso , Povo Asiático/genética , Calcifediol/sangue , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino , Doença de Parkinson/sangue
7.
J Microbiol Methods ; 119: 83-6, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26471200

RESUMO

Culture in enriched broth, as well as on a solid medium, is recommended for primary isolation of mycobacteria. With the introduction of liquid mycobacterial culture methods, a substantial workload regarding the identification of culture-recovered mycobacterial species, particularly Mycobacterium tuberculosis complex (MTC), has been imposed on our laboratory. We thus developed a triplex, real-time PCR coupled with pyrosequencing assay that can directly identify mycobacterial species from liquid media, which can reduce the workload. In this assay, real-time PCR simultaneously detects MTC and Mycobacterium xenopi, and amplifies the region of 16S rRNA gene containing hypervariable region A for pyrosequencing analysis; subsequent, pyrosequencing identifies many other nontuberculous mycobacteria. The assay was evaluated using 333 DNA samples directly prepared from liquid media, including 24 reference strains and 309 clinical isolates. Three hundred and twenty-eight (98.5%) of the 333 samples were correctly identified. The remaining five were determined as indeterminate. In conclusion, this coupled assay would be an alternative method for rapid identification of mycobacteria directly from liquid media in a clinical laboratory with a high workload in regions where tuberculosis is endemic.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala/métodos , Mycobacterium/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Tuberculose/microbiologia , DNA Bacteriano/genética , Feminino , Humanos , Masculino , Mycobacterium/genética , RNA Ribossômico 16S/genética
8.
Sensors (Basel) ; 14(8): 15163-81, 2014 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-25196015

RESUMO

Traffic patterns in wireless sensor networks (WSNs) usually follow a many-to-one model. Sensor nodes close to static sinks will deplete their limited energy more rapidly than other sensors, since they will have more data to forward during multihop transmission. This will cause network partition, isolated nodes and much shortened network lifetime. Thus, how to balance energy consumption for sensor nodes is an important research issue. In recent years, exploiting sink mobility technology in WSNs has attracted much research attention because it can not only improve energy efficiency, but prolong network lifetime. In this paper, we propose an energy efficient distance-aware routing algorithm with multiple mobile sink for WSNs, where sink nodes will move with a certain speed along the network boundary to collect monitored data. We study the influence of multiple mobile sink nodes on energy consumption and network lifetime, and we mainly focus on the selection of mobile sink node number and the selection of parking positions, as well as their impact on performance metrics above. We can see that both mobile sink node number and the selection of parking position have important influence on network performance. Simulation results show that our proposed routing algorithm has better performance than traditional routing ones in terms of energy consumption.


Assuntos
Redes de Comunicação de Computadores , Tecnologia de Sensoriamento Remoto , Tecnologia sem Fio , Algoritmos
9.
Diagn Microbiol Infect Dis ; 79(1): 49-53, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24565848

RESUMO

There are few commercial assays that easily and correctly identify the mycobacteria from culture in a clinical laboratory with a high workload. Thus, we developed and evaluated a scheme for the identification of mycobacteria using a multiplex real-time PCR assay and report on its application in our laboratory. The scheme consisted of 3 stepwise PCRs. Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) were differentially detected in the step 1 PCR, and the NTM species were identified in the step 2 and 3 PCRs. Over the 1.5-year study period, 1136 isolates of MTC and 618 isolates of NTM were detected, and the species of 608 (98.4%) of the 618 NTM isolates were identified. We conclude that the established scheme is a very useful diagnostic approach for the rapid and accurate identification of MTC and clinically relevant NTM in a clinical laboratory in a region where tuberculosis is endemic.


Assuntos
Tipagem Molecular/métodos , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/microbiologia , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Doenças Endêmicas , Humanos , Reação em Cadeia da Polimerase Multiplex , Infecções por Mycobacterium/epidemiologia , República da Coreia/epidemiologia
10.
J Clin Microbiol ; 51(3): 1008-13, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23269729

RESUMO

A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.


Assuntos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Infecções Estafilocócicas/diagnóstico , Proteínas de Bactérias/genética , Técnicas Bacteriológicas/métodos , Reações Falso-Positivas , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , Proteínas de Ligação às Penicilinas , RNA Ribossômico 16S/genética , Sensibilidade e Especificidade , Infecções Estafilocócicas/microbiologia
11.
J Clin Microbiol ; 50(2): 483-7, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22162553

RESUMO

A multiplex real-time PCR assay and melting curve analysis for identifying 23 mycobacterial species was developed and evaluated using 77 reference strains and 369 clinical isolates. Concordant results were obtained for all 189 (100%) isolates of the Mycobacterium tuberculosis complex and 169 (93.9%) isolates of nontuberculous mycobacteria. Our results showed that this multiplex real-time PCR assay is an effective tool for the mycobacterial identification from cultures.


Assuntos
Técnicas Bacteriológicas/métodos , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Mycobacterium tuberculosis/isolamento & purificação , Micobactérias não Tuberculosas/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Tuberculose/diagnóstico , Humanos , Mycobacterium tuberculosis/classificação , Mycobacterium tuberculosis/genética , Micobactérias não Tuberculosas/classificação , Micobactérias não Tuberculosas/genética , Temperatura de Transição
12.
Korean J Hematol ; 45(3): 150, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21120200
13.
Korean J Lab Med ; 30(2): 138-46, 2010 Apr.
Artigo em Coreano | MEDLINE | ID: mdl-20445331

RESUMO

BACKGROUND: We developed and evaluated the utility of a multiplex real-time PCR assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. METHODS: The specificity of the assay was tested using 4 reference strains of vancomycin-resistant enterococci (VRE) and 2 reference strains of vancomycin-susceptible enterococci. Ninety-three clinical isolates of enterococci with different glycopeptide-resistant phenotypes were genotyped and identified using a multiplex real-time PCR assay and melting curve analysis. RESULTS: Representative melting curves were obtained for Enterococcus faecium, Enterococcus faecalis, vanA-containing E. faecium, vanB-containing E. faecalis, Enterococcus gallinarum, and Enterococcus casseliflavus. Phenotypic and genotypic analysis of the isolates revealed same results for 82 enterococcal isolates, while in 4 isolates, the glycopeptide-resistant phenotypes were inconsistent with the glycopeptide-resistant genotypes and in the 4 other isolates, species could not be accurately identified. Three isolates with mixed strains, which were detected by the PCR assay, could not be correctly identified using phenotypic methods. CONCLUSIONS: VRE genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using multiplex real-time PCR assay and melting curve analysis.


Assuntos
Enterococcus/isolamento & purificação , Reação em Cadeia da Polimerase , Resistência a Vancomicina/genética , Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , DNA Bacteriano/genética , Enterococcus/genética , Enterococcus faecalis/genética , Enterococcus faecalis/isolamento & purificação , Enterococcus faecium/genética , Enterococcus faecium/isolamento & purificação , Genótipo , Desnaturação de Ácido Nucleico , Peptídeo Sintases/genética , Fenótipo
14.
J Korean Med Sci ; 25(4): 509-16, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20357989

RESUMO

The objective of this study was to identify the factors that determine serum homocysteine concentrations in Korean population. In a community-based study, 871 participants completed detailed questionnaires and physical examination. We found that increased age, male sex, family history of stroke, deficiencies of serum folate and vitamin B12, and elevated serum creatinine significantly increased the risk of hyperhomocysteinemia. However, hormonal and behavioral factors (smoking, alcohol drinking, coffee consumption, and sedentary time) were not associated with the risk of hyperhomocysteinemia. The risk of hyperhomocysteinemia was steeply increased in subjects with two or more risk factors among four selected risk factors (deficiencies of serum folate and vitamin B12, elevated creatinine, and family history of stroke) compared to subjects who did not have any risk factors, especially subjects over the age of 65 yr (odds ratio [OR], 33.5; 95% confidence interval [CI], 3.71-302.0 in men; OR, 39.2; 95% CI, 7.95-193.2 in women). In conclusion, increased age, male sex, family history of stroke, deficiencies of serum folate and vitamin B12, and elevated serum creatinine are important determinants of serum homocysteine concentrations with interaction effects between these factors.


Assuntos
Povo Asiático , Homocisteína/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Ácido Fólico/sangue , Humanos , Hiper-Homocisteinemia/sangue , Masculino , Pessoa de Meia-Idade , Razão de Chances , Fatores de Risco , Inquéritos e Questionários , Vitamina B 12/sangue , Adulto Jovem
15.
Sensors (Basel) ; 10(10): 9493-511, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-22163422

RESUMO

Energy efficiency and balancing is one of the primary challenges for wireless sensor networks (WSNs) since the tiny sensor nodes cannot be easily recharged once they are deployed. Up to now, many energy efficient routing algorithms or protocols have been proposed with techniques like clustering, data aggregation and location tracking etc. However, many of them aim to minimize parameters like total energy consumption, latency etc., which cause hotspot nodes and partitioned network due to the overuse of certain nodes. In this paper, a Distance-based Energy Aware Routing (DEAR) algorithm is proposed to ensure energy efficiency and energy balancing based on theoretical analysis of different energy and traffic models. During the routing process, we consider individual distance as the primary parameter in order to adjust and equalize the energy consumption among involved sensors. The residual energy is also considered as a secondary factor. In this way, all the intermediate nodes will consume their energy at similar rate, which maximizes network lifetime. Simulation results show that the DEAR algorithm can reduce and balance the energy consumption for all sensor nodes so network lifetime is greatly prolonged compared to other routing algorithms.


Assuntos
Algoritmos , Redes de Comunicação de Computadores , Processamento Eletrônico de Dados/métodos , Fontes Geradoras de Energia , Telemetria/métodos , Análise por Conglomerados , Simulação por Computador , Processamento Eletrônico de Dados/instrumentação , Telemetria/instrumentação
16.
Korean J Gastroenterol ; 50(5): 328-33, 2007 Nov.
Artigo em Coreano | MEDLINE | ID: mdl-18159166

RESUMO

Fitz-Hugh-Curtis syndrome, a kind of perihepatitis, occurs approximately in 3 to 10 percent of patients with pelvic inflammatory disease. It is not easy to detect in clinical settings due to requirement of invasive methods for diagnosis, for example, like a laparoscopic examination. Now, it has become possible to recognize it easily with the aid of non-invasive methods including an abdominal dynamic CT scan and laboratory tests. Moreover, it can be improved after the oral administration of antibiotics. Therefore, noninvasive diagnosis is desirable. Herein, clinical characteristics of ten cases of Fitz-Hugh-Curtis syndrome are reported, with a review of the literature.


Assuntos
Doença Inflamatória Pélvica/diagnóstico , Peritonite/diagnóstico , Adolescente , Adulto , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis , Diagnóstico Diferencial , Feminino , Humanos , Laparoscopia , Fígado/diagnóstico por imagem , Fígado/patologia , Doença Inflamatória Pélvica/tratamento farmacológico , Doença Inflamatória Pélvica/etiologia , Peritonite/tratamento farmacológico , Síndrome , Tomografia Computadorizada por Raios X
17.
Hypertension ; 45(5): 986-90, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15837835

RESUMO

The purpose of this pilot study was to test whether carvedilol has a protective effect against oxidative deoxyribonucleic acid (DNA) damage in human hypertension in vivo. Carvedilol's antioxidant effect has mostly focused on lipid or amino acid so far. However, there has been no data that carvedilol reduces DNA damage in human hypertension. Never-treated mild to moderate hypertension patients and age- and sex-matched control subjects volunteered for the study. The hypertension subjects were given 12.5 or 25 mg of carvedilol or hydrochlorothiazide orally for 2 months and controls were not given any. Fasting blood samples were collected before and after carvedilol. Plasma highly sensitive 8-hydroxy-2'-deoxyguanosine (hs8-OHdG) and high-sensitivity C-reactive protein (hsCRP) were checked with the samples. There were no statistical differences in clinical characteristics in 3 groups. The hs8-OHdG declined from 9.07+/-4.23 ng/mL to 5.74+/-3.89 ng/mL (P=0.002) after carvedilol. However, it did not show significant reduction after hydrochlorothiazide (9.01+/-3.89 versus 8.23+/-4.12 ng/mL; P=NS). In the control group, the hs8-OHdG concentration was 3.41+/-2.03 ng/mL and 3.01+/-2.65 ng/mL at baseline and 2 months later, respectively (P=NS). The baseline hs8-OHdG levels were higher in hypertension groups compared with control (P=0.000). The hsCRP had no significant difference before and after the tested drugs in 2 hypertension groups (group A: 0.21+/-0.51 versus 0.19+/-0.37 mg/dL; group B: 0.20+/-0.45 versus 0.18+/-0.42 mg/dL). In conclusion, DNA damage caused by reactive oxygen species occurs more in the hypertension patients than normals. Carvedilol significantly reduces DNA damage in the hypertension patients.


Assuntos
Antioxidantes/uso terapêutico , Carbazóis/uso terapêutico , Desoxiguanosina/análogos & derivados , Hipertensão/sangue , Hipertensão/tratamento farmacológico , Propanolaminas/uso terapêutico , 8-Hidroxi-2'-Desoxiguanosina , Adulto , Anti-Hipertensivos/uso terapêutico , Proteína C-Reativa/metabolismo , Carvedilol , Dano ao DNA/efeitos dos fármacos , Desoxiguanosina/antagonistas & inibidores , Desoxiguanosina/sangue , Método Duplo-Cego , Feminino , Humanos , Hidroclorotiazida/uso terapêutico , Hipertensão/genética , Hipertensão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Índice de Gravidade de Doença
18.
Korean J Gastroenterol ; 45(2): 137-42, 2005 Feb.
Artigo em Coreano | MEDLINE | ID: mdl-15725719

RESUMO

Fitz-Hugh-Curtis syndrome has been defined as perihepatitis accompanying pelvic inflammatory disease caused by Neisseria gonorrhoeae and Chlamydia trachomatis. In the acute phase, patients usually complain of severe right upper quadrant pain of sudden onset. The pain is sharp, pleuritic and most intense at the level of the right lower rib margin and thus it is frequently confused with acute cholecystitis or pleurisy. Definitive diagnosis of Fitz-Hugh-Curtis syndrome needs invasive procedures such as laparoscopy or laparotomy, but considering that Fitz-Hugh-Curtis syndrome is a benign condition that can be cured by oral administration of appropriate antibiotics, noninvasive diagnosis is desirable. Recently, we have experienced two cases of Fitz-Hugh-Curtis syndrome in acute phase accompanied with sharp and pleuritic right upper quadrant pain. In one case, pelvic inflammatory disease was not definite, so at first we mistook it for acute cholecystitis and reactivation of chronic hepatitis B. In the other case, Fitz-Hugh-Curtis syndrome followed the preceding, typical pelvic inflammatory disease. Both cases were diagnosed noninvasively and treated successfully by oral administration of antibiotics.


Assuntos
Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis , Gonorreia/diagnóstico , Hepatite/diagnóstico , Doença Inflamatória Pélvica/diagnóstico , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Síndrome
19.
J Korean Med Sci ; 17(4): 524-9, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12172050

RESUMO

This study was to clarify whether Behcet's disease (BD) could be classified into the spondyloarthropathy (SpA) complex. It was undertaken on 58 patients with BD (BD group), 56 patients with SpA (SpA group), and 3 patients who concurrently satisfied the criteria for BD and SpA (BDSpA group). The clinical parameters and known susceptible HLA antigens were compared between BD group and SpA group. In addition, 3 patients in BDSpA group were reviewed. The prevalence of definitive sacroiliitis (SI) in BD group and SpA group was 46.4% and 5.2%, respectively. However, none had a definitive SI in healthy controls. Enthesitis was observed in 3.4% of BD group and in 50% of SpA group. The patterns of eye involvement were different between these two groups. HLA-B27 was negative in all 49 patients of BD group, whereas it was positive in 67.9% of SpA group. The prevalence of HLA-B51 was 51.7% in BD group, and that in SpA group was 21.4%. One patient in BDSpA group was considered to have concurrent BD and ankylosing spondylitis (AS). Another patient was closer to AS, and the third to BD. Conclusively, it seems that BD could not be classified into the SpA complex.


Assuntos
Síndrome de Behçet/classificação , Espondilartrite , Adulto , Síndrome de Behçet/imunologia , Síndrome de Behçet/patologia , Olho/patologia , Feminino , Antígenos HLA-B/análise , Antígenos HLA-B/imunologia , Antígeno HLA-B27/análise , Antígeno HLA-B27/imunologia , Antígeno HLA-B51 , Humanos , Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/patologia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Pelve , Traçadores Radioativos , Radiografia , Articulação Sacroilíaca/diagnóstico por imagem , Articulação Sacroilíaca/patologia , Espondilartrite/imunologia , Espondilartrite/patologia , Tomografia Computadorizada de Emissão de Fóton Único
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...